Parasitology Training Manual

Acid Fast/Iron Haematoxylin Stain Protocol

1. 70% alcohol........................................10 minutes
2. water..................................................2 minutes
3. Kinyoun................................................5 minutes
4. water..................................................30 seconds
5. water..................................................30 seconds
6. water..................................................1 minute
7. acid/alcohol...........................................8 minutes
8. acid/alcohol...........................................30 seconds
9. water..................................................30 seconds
10. water..................................................1 minute
11. Iron Haematoxylin......................................10 minutes
12. water..................................................1 minute
13. Picric acid............................................15 minutes
14. water..................................................30 seconds
15. water..................................................30 seconds
16. water..................................................5 minutes
17. 70% alcohol............................................3 minutes
18. 95% alcohol............................................2 minutes
19. 95% alcohol............................................2 minutes
20. 100% alcohol(absolute).................................2 minutes
21. xylene substitute......................................2 minutes
22. xylene substitute......................................2 minutes
23. Coverslip and dry