Parasitology Training Manual
Entamoeba coli
See images below- Entamoeba coli is considered a non-pathogen at this time.
- The trophozoite and cyst of E. coli are both slightly larger than E. histolytica.
- The trophozoite measuring from 18 to 28 microns and larger has 1 nucleous containing a large diffuse
karyosome. The peripheral chromatin is usually dense and irregular. The cytoplasm is usually rough
with few to many ingested debris.
- In the wet mount it is usually impossible to differentiate between E. coli trophozoites and those
of E. histolytica. A stained smear is required.
- The cysts measures from 12 to 25 microns and typically contains 8 nuclei with very diffuse karyosomes.
- The cyst may also contain chromatoidal bars with very irregular fragmented ends.
- Due to the size of the cyst and its density in a permanent stained smear it usually shrinks considerably.
- Hypernucleation is possible with as many as 16 or more nuclei present in one cyst.
Click on the image below to enlarge.
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Consulting
David M. Raymondo
MLT, CLS(m)
Professional Affiliation: Gamma-Dynacare Medical Laboratories, Ottawa, Ontario, Canada
Gamma-Dynacare is a Division of the Dynacare Health Group